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KMID : 0669519940040000059
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Journal of Health Science - Yonsei University
1994 Volume.4 No. 0 p.59 ~ p.63
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Studies on T₃ Antibody Immobilization for Radioimmunoassay Use
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Abstract
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A study on immobilization of T₃antibody onto the inner wall of polystyrene tubes has been carried out. Into a series of polystyrene tubes, a constant volume of alkaline glutaraldehyde solution was added to obtain glutaraldehyde coated tubes by self-polymerization. The tubes were then treated with dilute T₃antibody solution to react the aldehyde residues on the wall-surface with the amino groups in antibodies. Reaction parameter such as temperature, volume of the glutaraldehyde solution, pH, dilution ratio of the antibody solution etc. were controlled. The average range of B/T(%) under standard T₃dose of upto 3.90ng/ml was 35.9-54.7. The results indicated that the antibody coated tubes are applicable for solid phase T₃RIA under a cautious control of accuracy keeping relatively long incubation time. However, an increased amount of the immobilized antibody is further desirable to shorten the incubation time and to increase the assay sensitivity.
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